HPLC TECHNOLOGY

Tosoh's advanced HPLC systems are fully automated systems that rapidly and precisely separate hemoglobins found naturally in human blood. Suitable for high throughput HbA1c testing in the laboratory or clinic environment in addition to rapid hemoglobinopathy screening, our HPLC solutions provide a reliable, accurate and precise result at all times.

HPLC is considered the “Gold Standard” technology in the monitoring of diabetic patients over time, via the measurement of HbA1c (= glycated hemoglobin fraction). This technique was used in the ground breaking DCCT (Diabetes Control and Complications Trial) study.

Pre-determined windows are set in the software to detect the presence of Hemoglobins A1a, A1b, F, l-A1c, s-A1c and A0 with other windows allowing presumptive identification of various variant hemoglobins.

Tosoh G8 HPLC Analyzer »



HPLC Column


High Performance Liquid Chromatography (HPLC)

This technique is a form of column chromatography used frequently in biochemistry and analytical chemistry. It involves passing a mixture containing the “analyte” through a column (stationary phase), by a liquid (mobile phase) at high pressure.

Cation exchange chromatography is a process that allows the separation of the mixture based on the charge properties of the molecules in the mixture. Cation exchange chromatography retains analyte molecules based on coulombic (ionic) interactions.

The stationary phase surface displays negatively charged functional groups that interact with positively charged cations in the mixture.